sequenom massarray methylation analysis  (Sequenom)

Journal: Discover Oncology

Article Title: CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation

doi: 10.1007/s12672-024-01194-8

Figure Lengend Snippet: Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Agena MassARRAY® Methylation Analysis assessed the methylation level of CTHRC1 in the promoter region.

Techniques: Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Western Blot

Journal: BMC Cancer

Article Title: Androgen receptor expression predicts breast cancer survival: the role of genetic and epigenetic events

doi: 10.1186/1471-2407-12-132

Figure Lengend Snippet: DNA methylation of the AR gene is associated with loss of AR protein expression in breast cancer cells . (a ) DNA methylation status of breast cancer cell lines. Three regions of AR were assessed by MS-HRM, overlapping Regions 1 and 2 in the AR minimal promoter and Region 3 at the translational start site (refer to Figure 1). Region 4 was assessed by Sequenom MassARRAY. Data represent the average of two independent experiments. ( b ) AR mRNA expression in breast cancer cell lines was assessed by qPCR. Expression is shown relative to β-actin and bars represent the mean ± standard deviation of two independent experiments.

Article Snippet: Sequenom MassARRAY methylation analysis was performed as described previously [ ].

Techniques: DNA Methylation Assay, Expressing, Standard Deviation

Journal: BMC Cancer

Article Title: Androgen receptor expression predicts breast cancer survival: the role of genetic and epigenetic events

doi: 10.1186/1471-2407-12-132

Figure Lengend Snippet: DNA methylation of an 115 bp region in the AR promoter is not associated with loss of AR protein expression in a selective group of primary breast tumours . Methylation of six CpG dinucleotides in the AR promoter of AR positive (Pos) and negative (Neg) Grade III breast tumours as determined by Sequenom MassARRAY (Region 4, Figure 1). Due to cleavage patterns, the average methylation was determined for CpG dinucleotides 2 and 3. Each dot represents an individual patient. A methylation value of 1.0 indicates a fully methylated amplicon, while a value of 0.0 indicates a fully unmethylated amplicon. Horizontal lines represent average cohort methylation.

Article Snippet: Sequenom MassARRAY methylation analysis was performed as described previously [ ].

Techniques: DNA Methylation Assay, Expressing, Methylation, Amplification